Centro de Inmunoterapia Veterinaria

Parvo virus mutation-C-A PROBLEM PARVA MUNDIAL

Parvo virus mutation-CPV-2C-A PROBLEM MUNDIAL
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ARTICLES OF INTEREST
Parvo C-STUDY FOR BREEDERS AND OWNERS OF PETS

WORK DONE BY THE HATCHERY DIMAHO

Professionals and researchers consulted
Dr. Danilo Bucafusco - Chair of Virology, Faculty of Vet. De la UBA
Dr. Leonardo Minatel - Chair of Pathology, Fac Vet. De la UBA
Dr. Jose de la Torre - CONICET - CEVAN-FUND. Milstein
Dr. Marina Gallo Calderon - CONICET - CEVAN-FUND. Milstein
Keller Leticia Hess - CONICET - CEVAN-FUND. Milstein
Dr. Paul Maure - Direct the CIV (Center for Veterinary Immunotherapy)
Dr. Javier Giacossa (Pfizer Laboratory)
Dr. Sebastin Vinisky (Fort Dodge Lab)
TCO. Walter Comas (Lab Intervet)

THE DANGER of disinformation:

This work of gathering information, interviews and articles, is intended to breeders and pet owners. (Our colleagues)

INTRODUCTION:

When the month of March this year, we began to ask some vets on the existence of the Parvo C, and if there were cases in the country, the answer was that they were unaware of the existence of this new strain of canine parvo virus, in Argentina.
Perhaps due to those who choose not to ask, is possible. But the reality is that at that time had no more knowledge of the CPV-2C, as a cause of death in our country.
No clutch, Dr. Marina Gallo Calderon had isolated strains of Parvo C (CPV-2c) in late 2007 and early 2008 ..
The team was composed by Marina Gallo Calderon, Nora Mattion and José La Torre (Director of Thesis), CONICET virologists, Danilo Bucafusco, Chair of Virology, Faculty of Veterinary Medicine UBA, Ferdinand Fogel, the clinical area Faculty of Tandil and Patricia Remorini, Bayer's San Pablo, Brazil. The hierarchy of each of these researchers, support the results obtained
In a talk with Dr. Jose de la Torre, Dr. Calderón, Dr. Jennifer Keller Hess and his team of Cevan, CONICET, showed us his interest in broadcasting and to inform all sectors involved, on this new strain .
It should be noted as these researchers with very low presupusto make admirable work.

CONCLUSIONS

Considering that this virus is infecting and killing puppies for over 4 years in countries like Spain, USA, and Germany, among other people, why did they not see that in these circumstances and considering the degree of development, could "predict future in the next 2 years "based on the experience of others.
The "secrecy" is making farmers are reluctant to acknowledge this with or parvo tubieron C. But one of Llosa consulted acknowledged and mortality was 20% average. This varies by region, by the ambient temperature. In Spain and the U.S., where the style is marketed through Pet-Shop, the mortality is much greater due to the high concentration of puppies, and the different background of each. For example in Europe, from Eastern Europe, have a higher degree of infection.
The conclusions we arrive, after having several conversations and exchanges of emails with laboratories, researchers and CEVAN CONICET-UBA, and some veterinarians are:

1) PVC-2C, not only exists, but is clearly expanding and moving to the strains 2a) and 2b)
This shift does not imply, as was the case 2a) which replaced the PVC-2, but variants coexist 2a): 2b) and 2C. This shift may be due to either the replicative advantage to have the same, compared to the other strains, or not having the mothers and pups specific defenses.

2) The virus isolated by PCR, correspond both dogs vaccinated and non vaccinated, so that existing vaccines do not give the puppy or mothers-specific immunity. Dan immunity from PVC-2a and 2b), and also "something" against CPV-2c.

3) Laboratories, argue that their vaccines provide protection for the PVC-2C, by:

• Crossing the antiviral 2a and 2b, (Fort Dodge)
• by the original strain PVC-2, which in their opinion provide all mutations, (Pfizer)
• or have a new strain (C154), which is PVC-2C strain included. It is up to each veterinarian these considerations. (Intervet-Schering)
The fact that the study shows that the C affection indiscriminately Parvo vaccinated and whether to vaccinate dogs.

4) The symptoms are not the same as parvo 2-a) and 2-b). Although similar, less frequent vomiting, diarrhea often are not bleeding, and less dehydration, not necessary in many cases with normal hydration. In this regard, the lesions found by the Dr.Leonardo Minatel in autopsies, are not exactly equal to those of parvo a) and b). (See Chair of Pathology of the FV of the UBA)

5) Given the resistance of PVC to environmental factors and viral load may be a factor in getting sick puppies vaccinated or infected. is important to disinfect as much as possible the nursery and can be used bleach (sodium hypochlorite 1 / 30 parts water, or Virkon Trifectant for 10 minutes before joining another litter, peroximono potassium sulphate and formalin fumes.

6) The virus is spread mainly by the feces of sick dogs, oral contact with the healthy, the virus does not "fly" from one cage to another, but if it goes on clothes, shoes, hair hands of the farm staff and vector-like rodents, birds or insects. It is therefore very important not only DISINFECT, but to establish management protocols PUPPIES. As different staff may handle sick puppies healthy.


The latter alternative would identify as a possible defense of litters, raising them to an uncontaminated environment for 30 days, weaned, and out of danger of contamination.

Still, considering that it has found the death of puppies for parvo "C would have only 3 months and 2 vaccines, there is always the uncertainty of the exposure to take that puppy to the new virus and the possibility of infection, and in the hands of the new owner.

TREATMENT AND PREVENTION AGAINST parvo CPV-2 C
The main thing is to prevent our kennel not between the virus. Pair this are insufficient hygiene measures and prophylaxis. It is necessary for puppies defenses does not give the mother or vaccines. With windows, or walls are not for the virus.
On the death of a puppy, for suspicious symptoms should have a necropsy at the Faculty of Med, UBA (San Martin and Chorroarín) low cost and safe result. Pathology determine if it is parvo or not. After PCR, may choose to isolate (Virology), paying an additional fee.
PREVENTION
In the absence of a vaccine against the virus completely covers, and the lack of defenses of the mothers, the inevitable question at each meeting with CEVAN researchers of CONICET and the Chair of Virology, Faculty of Medicine of the UBA was recurring
What can be done to reduce infections and sick puppies are more likely to survive the treatment?
In general, the suggestions were, in addition to maintaining adequate disinfection and vaccination plan, the need to lift the puppy's defenses, to prevent infection or who is stronger in case of illness.
Perhaps the persistence or luck, helped us to meet Dr. Paul Maure, at a conference on parvo C
Speaking with him, besides learning a lot about the immune system of puppies, we could get exactly what we wanted:


ALTERNATIVE SUPPLEMENTS FOR THE PREVENTION AND TREATMENT parvo "C" (CPV-2C)

Products used to defend against parvovirus and other viral infections.

Interferon alpha - beta (IFN α-β) oral: using a combination of both interferons (IFNs) in a dose of 100 IU (50 of 50 IFN α and IFN β) can develop a strong non-specific mucosal immunity without observed absorption of the blood, which inhibits not attenuated virus vaccine, and entering subcutaneously and the response is mounted in the lymph nodes. In the first 15 hours of oral intake, any virus can not replicate easily in the front door (mouth, nose or conjunctiva of the eye). The use of oral IFNs allowed in the first 24 hours have been virostatics in all mucous cells and activated in the oropharynx travel to other mucosal surfaces (respiratory, eye, gastrointestinal, genital) and even skin.

Inmunoalfa ®: is a thermosetting powder that has a combination of IFNs and lectins that promote a vigorous innate immune response, can be mixed in the concentrated feed in any category, we suggest initially use in females with cubs to the foot, weaned pups and animal rearing. The CIV is in contact with a balanced company to develop a food that already has Inmunoalfa ® built in it. We finished the pilot phase that lasted for two years with very good results.

IFN injection (IFN α): the use of IFN α weekly in all animals of different categories lowers the viral circulation. It is also a therapeutic agent to treat sick animals or animals suspected of being infected. We should note that the IFN α IFNs unlike injection oral vaccine itself can interfere, thus its administration should be under veterinary supervisación.

Interleukin two (IL-2): Many scientific studies in the world in various species showed that the simultaneous application of IL2 vaccine antigens increased the power of the same at the time of mounting an effector immune response. For months, in different locations of our professionals CIV IL2 vaccinate puppies as a booster of the vaccine. Also incorporated various breeding practice. Along with IFN α injection is an important therapeutic tool in virus-infected animals, since IL2 is the principal agent of growth of lymphocytes and viruses cause lymphopenia.
PREVENTION:

After the 25 days. (Even lavtando)
½ ML of interferon alpha + beta orally per day
1 ml of Beta Interferon alfa-week IM injection
1 tablespoon Inmunoalfa median of 10 kg of feed.
1 ml of interleukin 2 with vaccine (the same power) in separate syringes
At 30 days, to complete weaning, and bathing the puppies Virlon-S (not damaged virucidal mucous membranes), to avoid any possible virus that is in the hair, legs etc.
Dry with a towel, and place them in a cage Aseptic 100% - As of this age (30 days) is when he starts the greatest risk. So hygiene protocols should be implemented, disinfection and handling of puppies, to avoid contamination with viruses, the cage where they are puppies.

TREATMENT OF INFECTED PUPPY

Continuously observe the puppies (3 times a day at least)
When you depart, be less active and do not eat, begin treatment immediately.
1) separates the puppy infected the rest of the litter
2) 2) give the puppy infected
3) 1 ml every 8 h. Oral Interferon
4) 1 ml of interferon injected per day
(Besides the trtamiento by Parvo a) or b) to determine your veterinarian)

THE REST OF THE LITTER (estubo THAT PUPPY IN CONTACT WITH INFECTED)
Interferon 1ML ORAL EVERY 8 HS
Interferon Injection ½ ML PER DAY